验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60301-1-Ig targets BID in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | BID fusion protein Ag21005 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | BH3 interacting domain death agonist |
别名 | BID, FP497, p11 BID, p22 BID |
计算分子量 | 22 kDa |
观测分子量 | 22 kDa |
GenBank蛋白编号 | BC009197 |
基因名称 | BID |
Gene ID (NCBI) | 637 |
RRID | AB_2881416 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P55957 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).
实验方案
Product Specific Protocols | |
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WB protocol for BID antibody 60301-1-Ig | Download protocol |
IHC protocol for BID antibody 60301-1-Ig | Download protocol |
IF protocol for BID antibody 60301-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |