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验证数据展示

  • WB analysis of Jurkat using HRP-60301

    WB analysis of Jurkat using HRP-60301

    Jurkat cells were subjected to SDS PAGE followed by western blot with HRP-60301 (BID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

HRP-conjugated BID Monoclonal antibody

BID Monoclonal Antibody for WB
Cat No. HRP-60301

Print datasheet

CloneNo. 2A2E5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

BID, FP497, p11 BID, p22 BID

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-60301 targets BID in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen BID fusion protein Ag21005 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BH3 interacting domain death agonist
Synonyms BID, FP497, p11 BID, p22 BID
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
RRIDAB_2883832
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55957
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).

实验方案

Product Specific Protocols
WB protocol for HRP BID antibody HRP-60301Download protocol
Standard Protocols
Click here to view our Standard Protocols