• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD11a (TS2/4)

CD11a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/4

Cat no : 65194-1-Ig

Print datasheet

Synonyms

CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

验证数据展示

  • FC experiment of human PBMCs using 65194-1-Ig

    FC experiment of human PBMCs using 65194-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a (65194-1-Ig, Clone:TS2/4) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65194-1-Ig

    FC experiment of human PBMCs using 65194-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a (65194-1-Ig, Clone:TS2/4) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65194-1-Ig targets CD11a in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen HLA-DR CTL line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Synonyms CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A
Calculated Molecular Weight 129 kDa
GenBank Accession NumberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
RRIDAB_2918464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20701
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

实验方案

Product Specific Protocols
FC protocol for CD11a antibody 65194-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols