验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
CL488-65194 targets CD11a in IF, FC applications and shows reactivity with Human samples.
经测试应用 | FC Application Description |
文献引用应用 | IF |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | HLA-DR CTL line 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
别名 | CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A |
计算分子量 | 129 kDa |
GenBank蛋白编号 | BC008777 |
基因名称 | CD11a |
Gene ID (NCBI) | 3683 |
ENSEMBL Gene ID | ENSG00000005844 |
RRID | AB_2883216 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P20701 |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD11a antibody CL488-65194 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |