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Atlantic Blue™ Anti-Human CD11a (TS2/4)

CD11a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

TS2/4

Cat no : AB-65194

Print datasheet

Synonyms

CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

验证数据展示

  • FC experiment of human PBMCs using AB-65194

    FC experiment of human PBMCs using AB-65194

    1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD11a (AB-65194, Clone:TS2/4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using AB-65194

    FC experiment of human PBMCs using AB-65194

    1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD11a (AB-65194, Clone:TS2/4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

AB-65194 targets CD11a in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen HLA-DR CTL line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Synonyms CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A
Calculated Molecular Weight 129 kDa
GenBank Accession NumberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths404 nm / 458 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20701
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

实验方案

Product Specific Protocols
FC protocol for Atlantic Blue™ CD11a antibody AB-65194Download protocol
Standard Protocols
Click here to view our Standard Protocols