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CoraLite® Plus 405 Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody

CD20 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

2H7

Cat no : CL405-65575

Print datasheet

Synonyms

Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20

验证数据展示

  • FC experiment of human PBMCs using CL405-65575

    FC experiment of human PBMCs using CL405-65575

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19 and 5 ul CoraLite® Plus 405 Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody (CL405-65575, Clone: 2H7) (right), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL405-65575

    FC experiment of human PBMCs using CL405-65575

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody (CL405-65575, Clone: 2H7) (red), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65575-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65575 targets CD20 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human tonsillar B cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name membrane-spanning 4-domains, subfamily A, member 1
Synonyms Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20
Calculated Molecular Weight 297 aa, 33 kDa
GenBank Accession NumberBC002807
Gene Symbol CD20
Gene ID (NCBI) 931
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD20 antibody CL405-65575Download protocol
Standard Protocols
Click here to view our Standard Protocols