CoraLite® Plus 405 Anti-Human CD21 (BU32)
CD21 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 405 Fluorescent Dye
CloneNo.
BU32
验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 65198-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL405-65198 targets CD21 in FC applications and shows reactivity with Human samples.
Tested Applications | FC Application Description |
Tested Reactivity | Human |
Immunogen | N/A 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | complement component (3d/Epstein Barr virus) receptor 2 |
Synonyms | C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9 |
Calculated Molecular Weight | 1092 aa, 119 kDa |
GenBank Accession Number | BC136394 |
Gene Symbol | CD21 |
Gene ID (NCBI) | 1380 |
ENSEMBL Gene ID | ENSG00000117322 |
Conjugate | CoraLite® Plus 405 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P20023 |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 405 CD21 antibody CL405-65198 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |