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验证数据展示

  • FC experiment of human PBMCs using CL647-65198

    FC experiment of human PBMCs using CL647-65198

    1X10^6 human PBMCs were surface co-stained with FITC-CD19 and 5 ul CoraLite® Plus 647 Anti-Human CD21 (CL647-65198, Clone: BU32), or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65198

    FC experiment of human PBMCs using CL647-65198

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD21 (CL647-65198, Clone: BU32), or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

CoraLite® Plus 647 Anti-Human CD21 (BU32)

CD21 Monoclonal Antibody for FC
Cat No. CL647-65198

Print datasheet

CloneNo. BU32

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65198-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65198 targets CD21 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name complement component (3d/Epstein Barr virus) receptor 2
Synonyms C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9
Calculated Molecular Weight 1092 aa, 119 kDa
GenBank Accession NumberBC136394
Gene Symbol CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
RRIDAB_2883726
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20023
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD21 antibody CL647-65198Download protocol
Standard Protocols
Click here to view our Standard Protocols