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验证数据展示

  • FC experiment of human PBMCs using CL647-65198

    FC experiment of human PBMCs using CL647-65198

    1X10^6 human PBMCs were surface co-stained with FITC-CD19 and 5 ul CoraLite® Plus 647 Anti-Human CD21 (CL647-65198, Clone: BU32), or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65198

    FC experiment of human PBMCs using CL647-65198

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD21 (CL647-65198, Clone: BU32), or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

CoraLite® Plus 647 Anti-Human CD21 (BU32)

CD21 Monoclonal Antibody for FC
Cat No. CL647-65198

产品说明书

CloneNo. BU32

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65198-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65198 targets CD21 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component (3d/Epstein Barr virus) receptor 2
别名 C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9
计算分子量 1092 aa, 119 kDa
GenBank蛋白编号BC136394
基因名称 CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
RRIDAB_2883726
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20023
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD21 antibody CL647-65198Download protocol
Standard Protocols
Click here to view our Standard Protocols