CoraLite®488-conjugated CD22 Monoclonal antibody
CD22 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF-P
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
7A2F1
验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66103-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-66103 is CD22 Fusion Protein expressed in E. coli.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human |
| Immunogen | CD22 fusion protein Ag17986 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD22 molecule |
| Synonyms | B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14 |
| Calculated Molecular Weight | 847 aa, 95 kDa |
| GenBank Accession Number | BC109306 |
| Gene Symbol | CD22 |
| Gene ID (NCBI) | 933 |
| RRID | AB_2883259 |
| Conjugate | CoraLite®488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 491 nm / 516 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20273 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL488 CD22 antibody CL488-66103 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |