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CoraLite®594-conjugated CD22 Monoclonal antibody

CD22 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

7A2F1

Cat no : CL594-66103

Print datasheet

Synonyms

B cell receptor CD22, BL CAM, CD22, CD22 molecule, FLJ22814, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14

验证数据展示

  • IF Staining of human tonsillitis using CL594-66103

    IF Staining of human tonsillitis using CL594-66103

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66103 (CD22 antibody) at dilution of 1:100.

  • IF Staining of human tonsillitis using CL594-66103

    IF Staining of human tonsillitis using CL594-66103

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66103 (CD22 antibody) at dilution of 1:100.

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66103-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66103 is CD22 Fusion Protein expressed in E. coli.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen CD22 fusion protein Ag17986 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD22 molecule
Synonyms B cell receptor CD22, BL CAM, CD22, CD22 molecule, FLJ22814, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14
Calculated Molecular Weight 847 aa, 95 kDa
GenBank Accession NumberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
RRIDAB_2883513
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20273
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. And this antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 CD22 antibody CL594-66103Download protocol
Standard Protocols
Click here to view our Standard Protocols