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Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

BC96

Cat No : 65096-1-Ig

Print datasheet

Synonyms

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

验证数据展示

  • FC experiment of human PBMCs using 65096-1-Ig

    FC experiment of human PBMCs using 65096-1-Ig

    1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (right) or Mouse IgG1 Isotype Control (left). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (center). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 followed by CL488-conjugated Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65096-1-Ig

    FC experiment of human PBMCs using 65096-1-Ig

    1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (black dashed). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 647 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

65096-1-Ig targets CD25 in FC applications and shows reactivity with Human samples.

Tested Applications FC
Cited ApplicationsFC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 2 receptor, alpha
Synonyms CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR
Calculated Molecular Weight 272 aa, 31 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
RRIDAB_2918398
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01589
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for CD25 antibody 65096-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

DNA Cell Biol

Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.

Authors - Yuan-Hao Wu
View Article