Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

Conjugate

Unconjugated

Publications

CloneNo.

BC96

Cat no : 65096-1-Ig

Print datasheet

Synonyms

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

验证数据展示

FC experiment of human PBMCs using 65096-1-Ig
1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (right) or Mouse IgG1 Isotype Control (left). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (center). All samples were then stained with CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 followed by CL488-conjugated Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.
×
1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (right) or Mouse IgG1 Isotype Control (left). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (center). All samples were then stained with CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 followed by CL488-conjugated Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65096-1-Ig
1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (black dashed). All samples were then stained with CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
×
1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (black dashed). All samples were then stained with CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Cat No. 65096-1-Ig

Size

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经过测试的应用

Positive FC detected in

human PBMCs

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FC	See 1 publications below

产品信息

65096-1-Ig targets CD25 in FC applications and shows reactivity with Human samples.

Tested Applications	FC Application Description
Cited Applications	FC
Tested Reactivity	Human
Cited Reactivity	human
Immunogen	N/A 种属同源性预测
Host / Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Full Name	interleukin 2 receptor, alpha
Synonyms	CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR
Calculated Molecular Weight	272 aa, 31 kDa
GenBank Accession Number	BC156627
Gene Symbol	IL2RA
Gene ID (NCBI)	3559
RRID	AB_2918398
Conjugate	Unconjugated
Form	Liquid
Purification Method	Affinity purification
UNIPROT ID	P01589
Storage Buffer	PBS with 0.09% sodium azide.
Storage Conditions	Store at 2-8°C. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for CD25 antibody 65096-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	FC	DNA Cell Biol Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis. Authors - Yuan-Hao Wu View Article
		Allergy Exploring CD26-/lo subpopulations of lymphocytes in asthma phenotype and severity: A novel CD4+ T cell subset expressing archetypical granulocyte proteins Authors - Sara Vázquez-Mera View Article

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研究领域

Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

验证数据展示

FC experiment of human PBMCs using 65096-1-Ig

FC experiment of human PBMCs using 65096-1-Ig

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.

Exploring CD26-/lo subpopulations of lymphocytes in asthma phenotype and severity: A novel CD4+ T cell subset expressing archetypical granulocyte proteins

Mouse IgG1 Isotype Control (MOPC-21)

Mouse IgG1 isotype control Monoclonal antibody