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CoraLite® Plus 555 Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

BC96

Cat no : CL555-65096

Print datasheet

Synonyms

IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha, IL 2 RA

验证数据展示

  • FC experiment of human PBMCs using CL555-65096

    FC experiment of human PBMCs using CL555-65096

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD25 (CL555-65096, Clone:BC96) (red) or CoraLite® Plus 555 Mouse IgG1 Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD25 (CL555-65096, Clone:BC96) (black dashed). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL555-65096

    FC experiment of human PBMCs using CL555-65096

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD25 (CL555-65096, Clone:BC96) (right) or CoraLite® Plus 555 Mouse IgG1 Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD25 (CL555-65096, Clone:BC96) (center). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

经过测试的应用

Positive FC detected inPHA treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65096-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65096 targets CD25 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 2 receptor, alpha
Synonyms IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha, IL 2 RA
Calculated Molecular Weight 272 aa, 31 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01589
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD25 antibody CL555-65096Download protocol
Standard Protocols
Click here to view our Standard Protocols