• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 750 Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

BC96

Cat no : CL750-65096

Print datasheet

Synonyms

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

验证数据展示

  • FC experiment of human PBMCs using CL750-65096

    FC experiment of human PBMCs using CL750-65096

    1X10^6 PHA treated human PBMCs were surface co-stained with FITC Anti-Human CD4 and 5 ul CoraLite® Plus 750 Anti-Human CD25 (CL750-65096, Clone:BC96) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL750-65096

    FC experiment of human PBMCs using CL750-65096

    1X10^6 day 3 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD25 (CL750-65096, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). Unactivated PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD25 (black). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inPHA treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65096-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-65096 targets CD25 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 2 receptor, alpha
Synonyms CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR
Calculated Molecular Weight 272 aa, 31 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01589
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 CD25 antibody CL750-65096Download protocol
Standard Protocols
Click here to view our Standard Protocols