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Species Reactivity
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 98042-1-RR

    FC experiment of human PBMCs using 98042-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98042-1-RR

    FC experiment of human PBMCs using 98042-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98042-1-RR

    Affinity and Kinetic Characterization of 98042-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98042-1-RR against Human CD4 were performed. The affinity constant is 3.88 nM.

Anti-Human CD4 Rabbit Recombinant Antibody

CD4 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98042-1-RR

产品说明书

CloneNo. 240427E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98042-1-RR targets CD4 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD4 molecule
别名 T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12
计算分子量 55 kDa
GenBank蛋白编号BC025782
基因名称 CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_3672189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01730
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CD4 antibody 98042-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols