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Anti-Human CD4 Rabbit Recombinant Antibody

CD4 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240427E12

Cat no : 98042-1-RR

Print datasheet

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

验证数据展示

  • FC experiment of human PBMCs using 98042-1-RR

    FC experiment of human PBMCs using 98042-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98042-1-RR

    FC experiment of human PBMCs using 98042-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98042-1-RR

    Affinity and Kinetic Characterization of 98042-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98042-1-RR against Human CD4 were performed. The affinity constant is 3.88 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98042-1-RR is CD4 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD4 molecule
Synonyms T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01730
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CD4 antibody 98042-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols