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Anti-Human CD62L (DREG56)

CD62L Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

DREG56

Cat No : 65167-1-Ig

Print datasheet

Synonyms

L-selectin, L selectin, hLHRc, gp90-MEL, gp90 MEL

验证数据展示

  • FC experiment of human PBMCs using 65167-1-Ig

    FC experiment of human PBMCs using 65167-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD62L (65167-1-Ig, Clone:DREG56) or Mouse IgG1 Isotype Control and then CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with CL488 Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65167-1-Ig targets CD62L in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen PMA-activated human peripheral blood leukocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name selectin L
Synonyms L-selectin, L selectin, hLHRc, gp90-MEL, gp90 MEL
Calculated Molecular Weight 42 kDa
GenBank Accession NumberBC020758
Gene Symbol CD62L
Gene ID (NCBI) 6402
ENSEMBL Gene IDENSG00000188404
RRIDAB_2918444
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP14151
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

实验方案

Product Specific Protocols
FC protocol for CD62L antibody 65167-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols