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CoraLite® Plus 555 Anti-Human CD62L (DREG56)

CD62L Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

DREG56

Cat no : CL555-65167

Print datasheet

Synonyms

CD62L, gp90 MEL, hLHRc, L selectin, LAM 1, LAM1, LECAM1, Leu 8, Leukocyte adhesion molecule 1, LNHR, LSEL, Lyam 1, LYAM1, Lymph node homing receptor, PLNHR, selectin L, SELL, TQ1

验证数据展示

  • FC experiment of human PBMCs using CL555-65167

    FC experiment of human PBMCs using CL555-65167

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD62L (CL555-65167, Clone: DREG56) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL555-65167

    FC experiment of human PBMCs using CL555-65167

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD62L (CL555-65167, Clone: DREG56) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65167-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65167 targets CD62L in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen PMA-activated human peripheral blood leukocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name selectin L
Synonyms CD62L, gp90 MEL, hLHRc, L selectin, LAM 1, LAM1, LECAM1, Leu 8, Leukocyte adhesion molecule 1, LNHR, LSEL, Lyam 1, LYAM1, Lymph node homing receptor, PLNHR, selectin L, SELL, TQ1
Calculated Molecular Weight 42 kDa
GenBank Accession NumberBC020758
Gene Symbol CD62L
Gene ID (NCBI) 6402
ENSEMBL Gene IDENSG00000188404
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP14151
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD62L antibody CL555-65167Download protocol
Standard Protocols
Click here to view our Standard Protocols