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CoraLite® Plus 594 Anti-Human CD62L (DREG56)

CD62L Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

DREG56

Cat no : CL594-65167

Print datasheet

Synonyms

L-selectin, L selectin, hLHRc, gp90-MEL, gp90 MEL

验证数据展示

  • FC experiment of human PBMCs using CL594-65167

    FC experiment of human PBMCs using CL594-65167

    1x10^6 human PBMCs were surface stained with Atlantic Blue™ Anti-Human CD3 (AB-65151, Clone: UCHT1) and 5 ul CoraLite® Plus 594 Anti-Human CD62L (CL594-65167, Clone:DREG56) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL594-65167

    FC experiment of human PBMCs using CL594-65167

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD62L (CL594-65167, Clone:DREG56) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65167-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65167 targets CD62L in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen PMA-activated human peripheral blood leukocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name selectin L
Synonyms L-selectin, L selectin, hLHRc, gp90-MEL, gp90 MEL
Calculated Molecular Weight 42 kDa
GenBank Accession NumberBC020758
Gene Symbol CD62L
Gene ID (NCBI) 6402
ENSEMBL Gene IDENSG00000188404
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP14151
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 CD62L antibody CL594-65167Download protocol
Standard Protocols
Click here to view our Standard Protocols