Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10529-1-AP

Print datasheet

Synonyms

CHGA, CGA, ER-37, EA-92, Chromogranin-A



经过测试的应用

Positive WB detected inPC-12 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue, mouse small intestine tissue
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10529-1-AP targets Chromogranin A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Chromogranin A fusion protein Ag0807 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromogranin A (parathyroid secretory protein 1)
Synonyms CHGA, CGA, ER-37, EA-92, Chromogranin-A
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC006459
Gene Symbol Chromogranin A
Gene ID (NCBI) 1113
RRIDAB_2081122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
WB protocol for Chromogranin A antibody 10529-1-APDownload protocol
IHC protocol for Chromogranin A antibody 10529-1-APDownload protocol
IF protocol for Chromogranin A antibody 10529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Metab

Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance.

Authors - Yanru Wu
humanWB

Cell Biosci

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

Authors - Qiong Wang
humanWB

Sci Rep

Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line.

Authors - Azita Monazzam
humanWB

Discov Oncol

Depiction of neuroendocrine features associated with immunotherapy response using a novel one-class predictor in lung adenocarcinoma

Authors - Hao Liu
mouseIHC

Dose Response

The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury.

Authors - Danjie Liu
humanWB

Bioengineered

A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.

Authors - Zhirui Zeng