验证数据展示
经过测试的应用
Positive WB detected in | PC-12 cells |
Positive IHC detected in | human lung cancer tissue, human colon tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse pancreas tissue, mouse small intestine tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10529-1-AP targets Chromogranin A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | Chromogranin A fusion protein Ag0807 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | chromogranin A (parathyroid secretory protein 1) |
别名 | CHGA, CGA, ER-37, EA-92, Chromogranin-A |
计算分子量 | 51 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC006459 |
基因名称 | Chromogranin A |
Gene ID (NCBI) | 1113 |
RRID | AB_2081122 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P10645 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
实验方案
Product Specific Protocols | |
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WB protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
IHC protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
IF protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance. | ||
Cell Biosci Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer | ||
Sci Rep Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line. | ||
Discov Oncol Depiction of neuroendocrine features associated with immunotherapy response using a novel one-class predictor in lung adenocarcinoma | ||
Dose Response The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury. | ||
Bioengineered A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma. |