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验证数据展示

  • IF Staining of mouse pancreas using CL488-10529

    IF Staining of mouse pancreas using CL488-10529

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

  • IF Staining of mouse pancreas using CL488-10529

    IF Staining of mouse pancreas using CL488-10529

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

  • FC experiment of Neuro-2a using CL488-10529

    FC experiment of Neuro-2a using CL488-10529

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Chromogranin A (CL488-10529) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Neuro-2a using CL488-10529

    IF Staining of Neuro-2a using CL488-10529

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

CoraLite® Plus 488-conjugated Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL488-10529

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P, FC (Intra) and More (1)

别名

CHGA, CGA, ER-37, EA-92, Chromogranin-A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse pancreas tissue
Positive IF/ICC detected inNeuro-2a cells
Positive FC (Intra) detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10529-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-10529 targets Chromogranin A in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 Chromogranin A fusion protein Ag0807 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromogranin A (parathyroid secretory protein 1)
别名 CHGA, CGA, ER-37, EA-92, Chromogranin-A
计算分子量 51 kDa
GenBank蛋白编号BC006459
基因名称 Chromogranin A
Gene ID (NCBI) 1113
RRIDAB_2883077
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10645
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Chromogranin A antibody CL488-10529Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

bioRxiv

Pathobiont-triggered induction of epithelial IDO1 drives regional susceptibility to Inflammatory Bowel Disease

Authors - Paige N Spencer
View Article