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CoraLite® Plus 488-conjugated CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16686

Print datasheet

Synonyms

p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein

验证数据展示

  • IF Staining of HepG2 using CL488-16686

    IF Staining of HepG2 using CL488-16686

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CKAP4 antibody (CL488-16686) at dilution of 1:200.

  • FC experiment of HeLa using CL488-16686

    FC experiment of HeLa using CL488-16686

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CKAP4 (CL488-16686) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CKAP4 Ready-To-Use IHC Kit. CKAP4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16686-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16686 targets CKAP4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CKAP4 fusion protein Ag10019 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytoskeleton-associated protein 4
Synonyms p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC082972
Gene Symbol CKAP4
Gene ID (NCBI) 10970
RRIDAB_2919140
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07065
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CKAP4 antibody CL488-16686Download protocol
FC protocol for CL Plus 488 CKAP4 antibody CL488-16686Download protocol
Standard Protocols
Click here to view our Standard Protocols