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CoraLite®594-conjugated CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16686

Print datasheet

Synonyms

p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein

验证数据展示

  • IF Staining of HepG2 using CL594-16686

    IF Staining of HepG2 using CL594-16686

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 CKAP4 antibody (CL594-16686) at dilution of 1:200.

  • FC experiment of HeLa using CL594-16686

    FC experiment of HeLa using CL594-16686

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human CKAP4 (CL594-16686) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16686-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-16686 targets CKAP4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CKAP4 fusion protein Ag10019 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytoskeleton-associated protein 4
Synonyms p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC082972
Gene Symbol CKAP4
Gene ID (NCBI) 10970
RRIDAB_2919837
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07065
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

实验方案

Product Specific Protocols
IF protocol for CL594 CKAP4 antibody CL594-16686Download protocol
FC protocol for CL594 CKAP4 antibody CL594-16686Download protocol
Standard Protocols
Click here to view our Standard Protocols