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验证数据展示

  • IF Staining of HepG2 using CL594-16686

    IF Staining of HepG2 using CL594-16686

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 CKAP4 antibody (CL594-16686) at dilution of 1:200.

  • FC experiment of HeLa using CL594-16686

    FC experiment of HeLa using CL594-16686

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human CKAP4 (CL594-16686) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-16686

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CKAP4 Ready-To-Use IHC Kit. CKAP4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16686-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-16686 targets CKAP4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CKAP4 fusion protein Ag10019 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytoskeleton-associated protein 4
别名 p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein
计算分子量 602 aa, 66 kDa
观测分子量 63 kDa
GenBank蛋白编号BC082972
基因名称 CKAP4
Gene ID (NCBI) 10970
RRIDAB_2919837
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07065
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

实验方案

Product Specific Protocols
IF protocol for CL594 CKAP4 antibody CL594-16686Download protocol
FC protocol for CL594 CKAP4 antibody CL594-16686Download protocol
Standard Protocols
Click here to view our Standard Protocols