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验证数据展示

  • IF Staining of HeLa using CL594-12780

    IF Staining of HeLa using CL594-12780

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 CP110 antibody (CL594-12780) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CP110 Polyclonal antibody

CP110 Polyclonal Antibody for IF/ICC
Cat No. CL594-12780

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Centrosomal protein of 110 kDa, CP110, CP110 protein, DKFZp781G1416, KIAA0419

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CP110 Ready-To-Use IHC Kit. CP110 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12780-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-12780 targets CP110 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag3489

Product name: Recombinant human CP110 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-337 aa of BC036654

Sequence: MEEYEKFCEKSLARIQEASLSTESFLPAQSESISLIRFHGVAILSPLLNIEKRKEMQQEKQKALDVEARKQVNRKKALLTRVQEILDNVQVRKAPNASDFDQWEMETVYSNSEVRNLNVPATFPNSFPSHTEHSTAAKLDKIAGILPLDNEDQCKTDGIDLARDSEGFNSPKQCDSSNISHVENEAFPKTSSATPQETLISDGPFSVNEQQDLPLLAEVIPDPYVMSLQNLMKKSKEYIEREQSRRSLRGSMNRIVNESHLDKEHDAVEVADCVKEKGQLTGKHCVSVIPDKPSLNKSNVLLQGASTQASSMSMPVLASFSKVDIPIRTGHPTVLES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CP110 protein
别名 Centrosomal protein of 110 kDa, CP110, CP110 protein, DKFZp781G1416, KIAA0419
计算分子量 991 aa, 111 kDa
观测分子量 113 kDa
GenBank蛋白编号BC036654
基因名称 CP110
Gene ID (NCBI) 9738
RRIDAB_2919787
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43303
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

实验方案

Product Specific Protocols
IF protocol for CL594 CP110 antibody CL594-12780Download protocol
Standard Protocols
Click here to view our Standard Protocols