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验证数据展示

  • FC experiment of HeLa using CL488-15184

    FC experiment of HeLa using CL488-15184

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CPT1A (CL488-15184) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-15184

    IF Staining of HepG2 using CL488-15184

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CPT1A antibody (CL488-15184) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CPT1A Polyclonal antibody

CPT1A Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15184

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

CPT1-L, CPT1L, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CPT1A Ready-To-Use IHC Kit. CPT1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15184-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15184 targets CPT1A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CPT1A fusion protein Ag7202 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carnitine palmitoyltransferase 1A (liver)
别名 CPT1-L, CPT1L, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform
计算分子量 88 kDa
观测分子量 86 kDa
GenBank蛋白编号BC000185
基因名称 CPT1A
Gene ID (NCBI) 1374
RRIDAB_2919112
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50416
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CPT1A antibody CL488-15184Download protocol
FC protocol for CL Plus 488 CPT1A antibody CL488-15184Download protocol
Standard Protocols
Click here to view our Standard Protocols