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CoraLite® Plus 488-conjugated Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12289

Print datasheet

Synonyms

ApoJalpha, Apo-J, APOJ, Apo J, Aging-associated gene 4 protein

验证数据展示

  • IF Staining of HeLa using CL488-12289

    IF Staining of HeLa using CL488-12289

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Clusterin antibody (CL488-12289) at dilution of 1:200.

  • IF Staining of human liver using CL488-12289

    IF Staining of human liver using CL488-12289

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Clusterin antibody (CL488-12289) at dilution of 1:100.

经过测试的应用

Positive IF-P detected inhuman liver tissue
Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12289-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12289 targets Clusterin in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human
Immunogen Clusterin fusion protein Ag2889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clusterin
Synonyms ApoJalpha, Apo-J, APOJ, Apo J, Aging-associated gene 4 protein
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10909
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Clusterin antibody CL488-12289Download protocol
Standard Protocols
Click here to view our Standard Protocols