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验证数据展示

  • IF Staining of HeLa using CL555-12289

    IF Staining of HeLa using CL555-12289

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 Clusterin antibody (CL555-12289) at dilution of 1:200.

CoraLite®555-conjugated Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL555-12289

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IF-P

Synonyms

AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver tissue
Positive IF/ICC detected inHeLa cells, human liver tissue
For other applications, we recommend the unconjugated version of this antibody, 12289-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-12289 targets Clusterin in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human
Immunogen Clusterin fusion protein Ag2889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clusterin
Synonyms AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
RRIDAB_3084527
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10909
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
IF protocol for CL555 Clusterin antibody CL555-12289Download protocol
Standard Protocols
Click here to view our Standard Protocols