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验证数据展示

  • IF Staining of HeLa using CL555-12289

    IF Staining of HeLa using CL555-12289

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 Clusterin antibody (CL555-12289) at dilution of 1:200.

CoraLite®555-conjugated Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL555-12289

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P

别名

AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver tissue
Positive IF/ICC detected inHeLa cells, human liver tissue
For other applications, we recommend the unconjugated version of this antibody, 12289-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-12289 targets Clusterin in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原 Clusterin fusion protein Ag2889 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clusterin
别名 AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2
计算分子量 449 aa, 53 kDa
观测分子量 37 kDa
GenBank蛋白编号BC010514
基因名称 CLU
Gene ID (NCBI) 1191
RRIDAB_3084527
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10909
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
IF protocol for CL555 Clusterin antibody CL555-12289Download protocol
Standard Protocols
Click here to view our Standard Protocols