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验证数据展示

  • IF Staining of HeLa using CL555-14965

    IF Staining of HeLa using CL555-14965

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 Cytokeratin 19 antibody (CL555-14965) at dilution of 1:200.

  • FC experiment of MCF-7 using CL555-14965

    FC experiment of MCF-7 using CL555-14965

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 19 (CL555-14965) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-14965

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14965-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14965 targets Cytokeratin 19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 19 fusion protein Ag6830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 19
别名 Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19
计算分子量 44 kDa
GenBank蛋白编号BC002539
基因名称 Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2919621
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08727
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19.

实验方案

Product Specific Protocols
IF protocol for CL555 Cytokeratin 19 antibody CL555-14965Download protocol
Standard Protocols
Click here to view our Standard Protocols