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CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17329

Print datasheet

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

验证数据展示

  • IF Staining of HT-29 using CL488-17329

    IF Staining of HT-29 using CL488-17329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-17329) at dilution of 1:200.

  • FC experiment of HT-29 using CL488-17329

    FC experiment of HT-29 using CL488-17329

    1x10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody (CL488-17329)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17329-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17329 targets Cytokeratin 20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen Cytokeratin 20 fusion protein Ag11308 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 20
Synonyms KRT20, keratin 20, K20, Cytokeratin-20, CK-20
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC031559
Gene Symbol Cytokeratin 20
Gene ID (NCBI) 54474
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35900
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329Download protocol
FC protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329Download protocol
Standard Protocols
Click here to view our Standard Protocols