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验证数据展示

  • IF Staining of HT-29 using CL488-17329

    IF Staining of HT-29 using CL488-17329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-17329) at dilution of 1:200.

  • FC experiment of HT-29 using CL488-17329

    FC experiment of HT-29 using CL488-17329

    1x10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody (CL488-17329)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-17329

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, rabbit

应用

IF/ICC, FC (Intra)

别名

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17329-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17329 targets Cytokeratin 20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 Cytokeratin 20 fusion protein Ag11308 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 20
别名 KRT20, keratin 20, K20, Cytokeratin-20, CK-20
计算分子量 424 aa, 48 kDa
观测分子量 46 kDa
GenBank蛋白编号BC031559
基因名称 Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_3084003
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35900
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329Download protocol
FC protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329Download protocol
Standard Protocols
Click here to view our Standard Protocols