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验证数据展示

  • IF Staining of HT-29 using CL594-60183

    IF Staining of HT-29 using CL594-60183

    Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using CoraLite®594 Cytokeratin 20 antibody (CL594-60183, Clone: 4D10A4 ) at dilution of 1:200.

CoraLite®594-conjugated Cytokeratin 20 Monoclonal antibody

Cytokeratin 20 Monoclonal Antibody for IF/ICC
Cat No. CL594-60183

Print datasheet

CloneNo. 4D10A4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60183-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60183 targets Cytokeratin 20 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Cytokeratin 20 fusion protein Ag11336 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 20
Synonyms Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT
Calculated Molecular Weight 424 aa, 48 kDa
GenBank Accession NumberBC031559
Gene Symbol Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_2919900
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35900
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytokeratin 20 antibody CL594-60183Download protocol
Standard Protocols
Click here to view our Standard Protocols