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CoraLite® Plus 488-conjugated Cytokeratin 20 Monoclonal antibody

Cytokeratin 20 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4D10A4

Cat no : CL488-60183

Print datasheet

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

验证数据展示

  • IF Staining of HT-29 using CL488-60183

    IF Staining of HT-29 using CL488-60183

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200.

  • IF Staining of human colon using CL488-60183

    IF Staining of human colon using CL488-60183

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200, CoraLite®594 CD68 antibody (CL594-25747, red).

  • IF Staining of human colon using CL488-60183

    IF Staining of human colon using CL488-60183

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200, CoraLite®594 CD68 antibody (CL594-25747, red).

经过测试的应用

Positive IF-P detected inhuman colon tissue
Positive IF/ICC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60183-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60183 targets Cytokeratin 20 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human
Immunogen Cytokeratin 20 fusion protein Ag11336 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 20
Synonyms KRT20, keratin 20, K20, Cytokeratin-20, CK-20
Calculated Molecular Weight 424 aa, 48 kDa
GenBank Accession NumberBC031559
Gene Symbol Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_2919234
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35900
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-60183Download protocol
Standard Protocols
Click here to view our Standard Protocols