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CoraLite® Plus 488-conjugated EEF1A1 Polyclonal antibody
EEF1A1 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy EEF1A1 Ready-To-Use IHC Kit. EEF1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11402-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-11402 targets EEF1A1 in IF applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | EEF1A1 fusion protein Ag1938 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | eukaryotic translation elongation factor 1 alpha 1 |
Synonyms | CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, GRAF 1EF, HNGC:16303, LENG7, PTI1 |
Calculated Molecular Weight | 462 aa, 50 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC014224 |
Gene Symbol | EEF1A1 |
Gene ID (NCBI) | 1915 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P68104 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 EEF1A1 antibody CL488-11402 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |