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验证数据展示

  • WB analysis using CL488-51071

    WB analysis using CL488-51071

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-51071 (EGFR (C-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using CL488-51071

    IF Staining of A431 using CL488-51071

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 EGFR (C-terminal) antibody (CL488-51071) at dilution of 1:100.

  • FC experiment of A431 using CL488-51071

    FC experiment of A431 using CL488-51071

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 EGFR (C-Terminal) Polyclonal Antibody (CL488-51071)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated EGFR (C-terminal) Polyclonal antibody

EGFR (C-terminal) Polyclonal Antibody for WB, IF/ICC, FC (Intra)
Cat No. CL488-51071

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra)

别名

EGFR, EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-51071 targets EGFR (C-terminal) in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 EGFR, EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1
计算分子量 1210 aa, 134 kDa
观测分子量 170 kDa
GenBank蛋白编号BC094761
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_3672851
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00533
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 EGFR (C-terminal) antibody CL488-51071Download protocol
IF protocol for CL Plus 488 EGFR (C-terminal) antibody CL488-51071Download protocol
FC protocol for CL Plus 488 EGFR (C-terminal) antibody CL488-51071Download protocol
Standard Protocols
Click here to view our Standard Protocols