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CoraLite® Plus 488-conjugated EZH2 Polyclonal antibody
EZH2 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HepG2 cells |
This antibody is not recommended for IHC.
For other applications, we recommend the unconjugated version of this antibody, 21800-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-21800 targets EZH2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | EZH2 fusion protein Ag16789 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | enhancer of zeste homolog 2 (Drosophila) |
Synonyms | KMT6, EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2 |
Calculated Molecular Weight | 751 aa, 86 kDa |
Observed Molecular Weight | 90-102 kDa |
GenBank Accession Number | BC010858 |
Gene Symbol | EZH2 |
Gene ID (NCBI) | 2146 |
RRID | AB_2919183 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q15910 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 EZH2 antibody CL488-21800 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |