Factor XIIIa Monoclonal antibody

Factor XIIIa Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66325-1-Ig

产品说明书

CloneNo. 2D2B2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, ELISA

F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inpig colon tissue, human placenta tissue, human peripheral blood platelets, rat colon tissue, rabbit large intestine tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU-937 cells
Planning an IHC experiment? We recommend our IHCeasy F13A1 Ready-To-Use IHC Kit. F13A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66325-1-Ig targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human
免疫原 Factor XIIIa fusion protein Ag11105 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor XIII, A1 polypeptide
别名 F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa
计算分子量 732 aa, 83 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC027963
基因名称 Factor XIIIa
Gene ID (NCBI) 2162
RRIDAB_2881706
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00488
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.

实验方案

Product Specific Protocols
WB protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
IHC protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Inflamm Regen

Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling

Authors - Hirofumi Watanabe
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