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验证数据展示

  • WB analysis using 66325-1-Ig (same clone as 66325-1-PBS)

    WB analysis using 66325-1-Ig (same clone as 66325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood platelets using 66325-1-Ig (same clone as 66325-1-PBS)

    WB analysis of human peripheral blood platelets using 66325-1-Ig (same clone as 66325-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit large intestine using 66325-1-Ig (same clone as 66325-1-PBS)

    WB analysis of rabbit large intestine using 66325-1-Ig (same clone as 66325-1-PBS)

    rabbit large intestine tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 66325-1-Ig (same clone as 66325-1-PBS)

    WB analysis of human placenta using 66325-1-Ig (same clone as 66325-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

    IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with F13A1 antibody included
  • IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

    IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with F13A1 antibody included
  • WB analysis of rat colon using 66325-1-Ig (same clone as 66325-1-PBS)

    WB analysis of rat colon using 66325-1-Ig (same clone as 66325-1-PBS)

    rat colon tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

  • FC experiment of U-937 using 66325-1-Ig (same clone as 66325-1-PBS)

    FC experiment of U-937 using 66325-1-Ig (same clone as 66325-1-PBS)

    1x10^6 U-937 cells were intracellularly stained with 0.25 ug Factor XIIIa Monoclonal antibody (66325-1-Ig, Clone:2D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

Factor XIIIa Monoclonal antibody, PBS Only

Factor XIIIa Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66325-1-PBS

产品说明书

CloneNo. 2D2B2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, Indirect ELISA

别名

F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy F13A1 Ready-To-Use IHC Kit. F13A1 primary antibody included.

产品信息

66325-1-PBS targets Factor XIIIa in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag11105

Product name: Recombinant human F13A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 383-732 aa of BC027963

Sequence: RPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAIKHGHVCFQFDAPFVFAEVNSDLIYITAKKDGTHVVENVDATHIGKLIVTKQIGGDGMMDITDTYKFQEGQEEERLALETALMYGAKKPLNTEGVMKSRSNVDMDFEVENAVLGKDFKLSITFRNNSHNRYTITAYLSANITFYTGVPKAEFKKETFDVTLEPLSFKKEAVLIQAGEYMGQLLEQASLHFFVTARINETRDVLAKQKSTVLTIPEIIIKVRGTQVVGSDMTVIVEFTNPLKETLRNVWVHLDGPGVTRPMKKMFREIRPNSTVQWEEVCRPWVSGHRKLIASMSSDSLRHVYGELDVQIQRRPSM

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor XIII, A1 polypeptide
别名 F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa
计算分子量 732 aa, 83 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC027963
基因名称 Factor XIIIa
Gene ID (NCBI) 2162
RRIDAB_2881706
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00488
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.