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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Fibronectin Monoclonal antibody

Fibronectin Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10F9

Cat no : CL488-66042

Print datasheet

Synonyms

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

验证数据展示

  • IF Staining of NIH/3T3 using CL488-66042

    IF Staining of NIH/3T3 using CL488-66042

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CL488-66042 (Fibronectin antibody) at dilution of 1:100.

  • IF Staining of human colon cancer using CL488-66042

    IF Staining of human colon cancer using CL488-66042

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Fibronectin antibody (CL488-66042, Clone: 1G10F9 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 66042-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

CL488-66042 targets Fibronectin in IHC, IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Fibronectin fusion protein Ag8016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibronectin 1
Synonyms FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG
Calculated Molecular Weight 2386 aa, 263 kDa
Observed Molecular Weight 263 kDa
GenBank Accession NumberBC005858
Gene Symbol Fibronectin
Gene ID (NCBI) 2335
RRIDAB_2883236
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02751
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Fibronectin antibody CL488-66042Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Front Pharmacol

Ginsenoside Rb1 Ameliorates Diabetic Arterial Stiffening via AMPK Pathway.

Authors - Xinyu Zhang
View Article