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验证数据展示

  • IF Staining of human colon cancer using CL594-66042

    IF Staining of human colon cancer using CL594-66042

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-66042

    IF Staining of human colon cancer using CL594-66042

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

  • IF Staining of NIH/3T3 using CL594-66042

    IF Staining of NIH/3T3 using CL594-66042

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CL594-66042 (Fibronectin antibody) at dilution of 1:100.

  • FC experiment of HEK-293 using CL594-66042

    FC experiment of HEK-293 using CL594-66042

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Fibronectin (CL594-66042, Clone:1G10F9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Fibronectin Monoclonal antibody

Fibronectin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL594-66042

产品说明书

CloneNo. 1G10F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P, FC (Intra)

别名

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66042-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66042 targets Fibronectin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Fibronectin fusion protein Ag8016 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fibronectin 1
别名 FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG
计算分子量 2386 aa, 263 kDa
观测分子量 263 kDa
GenBank蛋白编号BC005858
基因名称 Fibronectin
Gene ID (NCBI) 2335
RRIDAB_2883488
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02751
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

实验方案

Product Specific Protocols
IF protocol for CL594 Fibronectin antibody CL594-66042Download protocol
FC protocol for CL594 Fibronectin antibody CL594-66042Download protocol
Standard Protocols
Click here to view our Standard Protocols