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CoraLite®594-conjugated Fibronectin Monoclonal antibody
Fibronectin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G10F9
验证数据展示
经过测试的应用
Positive IF-P detected in | human colon cancer tissue |
Positive IF/ICC detected in | NIH/3T3 cells |
Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66042 targets Fibronectin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IF-P, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Fibronectin fusion protein Ag8016 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | fibronectin 1 |
Synonyms | FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG |
Calculated Molecular Weight | 2386 aa, 263 kDa |
Observed Molecular Weight | 263 kDa |
GenBank Accession Number | BC005858 |
Gene Symbol | Fibronectin |
Gene ID (NCBI) | 2335 |
RRID | AB_2883488 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P02751 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL594 Fibronectin antibody CL594-66042 | Download protocol |
FC protocol for CL594 Fibronectin antibody CL594-66042 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |