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验证数据展示

  • WB analysis using CL488-66574

    WB analysis using CL488-66574

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66574 (GRP78/BIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GRP78/BIP Monoclonal antibody

GRP78/BIP Monoclonal Antibody for WB
Cat No. CL488-66574

Print datasheet

CloneNo. 1D6F7

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB

Synonyms

BIP, FLJ26106, GRP 78, GRP78, Heat shock 70 kDa protein 5, HSPA5, MIF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66574-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66574 targets GRP78/BIP in WB applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB Application Description
Tested Reactivity Human, rat, mouse
Immunogen GRP78/BIP fusion protein Ag2188 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms BIP, FLJ26106, GRP 78, GRP78, Heat shock 70 kDa protein 5, HSPA5, MIF2
Calculated Molecular Weight 78 kDa
Observed Molecular Weight78 kDa
GenBank Accession NumberBC020235
Gene Symbol GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2919345
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11021
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 GRP78/BIP antibody CL488-66574Download protocol
Standard Protocols
Click here to view our Standard Protocols