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验证数据展示

  • FC experiment of HeLa using CL647-66574

    FC experiment of HeLa using CL647-66574

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GRP78/BIP Monoclonal antibody (CL647-66574, Clone:1D6F7)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated GRP78/BIP Monoclonal antibody

GRP78/BIP Monoclonal Antibody for FC (Intra)
Cat No. CL647-66574

产品说明书

CloneNo. 1D6F7

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

FC (Intra)

别名

BIP, FLJ26106, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66574-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66574 targets GRP78/BIP in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat, mouse
免疫原 GRP78/BIP fusion protein Ag2188 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
别名 BIP, FLJ26106, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2
计算分子量 78 kDa
观测分子量 70-78 kDa
GenBank蛋白编号BC020235
基因名称 GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2935062
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP11021
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 GRP78/BIP antibody CL647-66574Download protocol
Standard Protocols
Click here to view our Standard Protocols