验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66574 targets GRP78/BIP in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | Human, rat, mouse |
免疫原 | GRP78/BIP fusion protein Ag2188 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
别名 | BIP, FLJ26106, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2 |
计算分子量 | 78 kDa |
观测分子量 | 70-78 kDa |
GenBank蛋白编号 | BC020235 |
基因名称 | GRP78/BIP |
Gene ID (NCBI) | 3309 |
RRID | AB_2935062 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P11021 |
储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 GRP78/BIP antibody CL647-66574 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |