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APC-Cyanine7 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

APC-Cyanine7 Fluorescent Dye

CloneNo.

L243

Cat no : AY7-65560

Print datasheet

Synonyms

HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA

验证数据展示

  • FC experiment of human PBMCs using AY7-65560

    FC experiment of human PBMCs using AY7-65560

    1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 HLA-DR Recombinant Antibody (AY7-65560, Clone: L243) or unstained. Cells were then stained with 5 ul CoraLite® Plus 488 Anti-Human CD19 (HIB19) (CL488-65110, Clone: HIB19). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

AY7-65560 targets HLA-DR in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human lymphoblastoid B-cell line RPMI 8866 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR alpha
Synonyms HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC032350
Gene Symbol HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
Conjugate APC-Cyanine7 Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 778 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01903
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for APC-Cyanine7 HLA-DR antibody AY7-65560Download protocol
Standard Protocols
Click here to view our Standard Protocols