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验证数据展示

  • FC experiment of human PBMCs using CL594-65560

    FC experiment of human PBMCs using CL594-65560

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14 and 5 ul CoraLite®594 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL594-65560, Clone: L243), or 5 ul CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL594-65560

    FC experiment of human PBMCs using CL594-65560

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL594-65560, Clone: L243) (red), or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite®594 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Uni-rAbTM Recombinant Antibody for FC
Cat No. CL594-65560

产品说明书

CloneNo. L243

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65560-1-MR

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65560 targets HLA-DR in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human lymphoblastoid B-cell line RPMI 8866 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 major histocompatibility complex, class II, DR alpha
别名 HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC032350
基因名称 HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01903
储存缓冲液 PBS with 0.09% sodium azide.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).

实验方案

Product Specific Protocols
FC protocol for CL594 HLA-DR antibody CL594-65560Download protocol
Standard Protocols
Click here to view our Standard Protocols