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CoraLite® Plus 647 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

L243

Cat No : CL647-65560

Print datasheet

Synonyms

HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA

验证数据展示

  • FC experiment of human PBMCs using CL647-65560

    FC experiment of human PBMCs using CL647-65560

    1x10^6 human PBMCs were surface stained with FITC Anti-Human CD19 and 5 ul CoraLite® Plus 647 Anti-Human HLA-DR Mouse Recombinant Antibody (CL647-65560, Clone:L243), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65560-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65560 targets HLA-DR in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human lymphoblastoid B-cell line RPMI 8866 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR alpha
Synonyms HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC032350
Gene Symbol HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01903
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 HLA-DR antibody CL647-65560Download protocol
Standard Protocols
Click here to view our Standard Protocols