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CoraLite® Plus 647 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

L243

Cat no : CL647-65560

Print datasheet

Synonyms

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

验证数据展示

  • FC experiment of human PBMCs using CL647-65560

    FC experiment of human PBMCs using CL647-65560

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD14 and 5 ul CoraLite® Plus 647 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL647-65560, Clone: L243), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL647-65560

    FC experiment of human PBMCs using CL647-65560

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL647-65560, Clone: L243) (red), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65560-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65560 targets HLA-DR in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human lymphoblastoid B-cell line RPMI 8866 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR alpha
Synonyms HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC032350
Gene Symbol HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01903
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 HLA-DR antibody CL647-65560Download protocol
Standard Protocols
Click here to view our Standard Protocols