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CoraLite® Plus 488-conjugated HLA class I ABC Monoclonal antibody
HLA class I ABC Monoclonal Antibody for IF/ICC, IF-P, FC
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
IF/ICC, IF-P, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5C5B7
验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue, Raji cells |
Positive IF/ICC detected in | Raji cells |
Positive FC detected in | Raji cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66013 targets HLA class I ABC in IF/ICC, IF-P, FC applications and shows reactivity with human, pig samples.
Tested Applications | IF/ICC, IF-P, FC Application Description |
Tested Reactivity | human, pig |
Immunogen | HLA class I ABC fusion protein Ag7488 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | major histocompatibility complex, class I, A |
Synonyms | HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 40-44 kDa |
GenBank Accession Number | BC003069 |
Gene Symbol | HLA-A |
Gene ID (NCBI) | 3105 |
RRID | AB_2883227 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04439 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HLA class I ABC antibody CL488-66013 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |