验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66013 targets HLA class I ABC in IF-P applications and shows reactivity with human, pig samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human, pig |
免疫原 | HLA class I ABC fusion protein Ag7488 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | major histocompatibility complex, class I, A |
别名 | HLA A, HLAA, HLA-A, MHC class I antigen A*3 |
计算分子量 | 41 kDa |
观测分子量 | 40-44 kDa |
GenBank蛋白编号 | BC003069 |
基因名称 | HLA-A |
Gene ID (NCBI) | 3105 |
RRID | AB_2883477 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P04439 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
实验方案
Product Specific Protocols | |
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IF protocol for CL594 HLA class I ABC antibody CL594-66013 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |