Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB result of HRAS-Specific antibody (18295-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRAS-Specific transfected HEK-293 cells.

Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with mouse brain tissue lysate 1840 ug.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HRAS-Specific antibody (18295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HRAS-Specific (18295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18295-1-AP (HRAS-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).