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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IFITM3 Polyclonal antibody

IFITM3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11714

Print datasheet

Synonyms

IP15, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b

验证数据展示

  • IF Staining of HeLa using CL488-11714

    IF Staining of HeLa using CL488-11714

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IFITM3 antibody (CL488-11714) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-11714

    FC experiment of HeLa using CL488-11714

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IFITM3 (CL488-11714) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFITM2/3 Ready-To-Use IHC Kit. IFITM2/3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11714-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11714 targets IFITM3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen IFITM3 fusion protein Ag2285 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon induced transmembrane protein 3 (1-8U)
Synonyms IP15, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b
Calculated Molecular Weight 133 aa, 15 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC006794
Gene Symbol IFITM3
Gene ID (NCBI) 10410
RRIDAB_2919038
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01628
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFITM3 antibody CL488-11714Download protocol
FC protocol for CL Plus 488 IFITM3 antibody CL488-11714Download protocol
Standard Protocols
Click here to view our Standard Protocols