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验证数据展示

  • IF Staining of HeLa using CL594-11714

    IF Staining of HeLa using CL594-11714

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 IFITM3 antibody (CL594-11714) at dilution of 1:200, CL488-phalloidin (green).

  • IF Staining of HeLa using CL594-11714

    IF Staining of HeLa using CL594-11714

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 IFITM3 antibody (CL594-11714) at dilution of 1:200, CL488-phalloidin (green).

  • FC experiment of HeLa using CL594-11714

    FC experiment of HeLa using CL594-11714

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human IFITM3 (CL594-11714) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated IFITM3 Polyclonal antibody

IFITM3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-11714

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

IP15, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFITM2/3 Ready-To-Use IHC Kit. IFITM2/3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11714-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-11714 targets IFITM3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 IFITM3 fusion protein Ag2285 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon induced transmembrane protein 3 (1-8U)
别名 IP15, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b
计算分子量 133 aa, 15 kDa
观测分子量 14 kDa
GenBank蛋白编号BC006794
基因名称 IFITM3
Gene ID (NCBI) 10410
RRIDAB_2919772
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01628
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

实验方案

Product Specific Protocols
IF protocol for CL594 IFITM3 antibody CL594-11714Download protocol
FC protocol for CL594 IFITM3 antibody CL594-11714Download protocol
Standard Protocols
Click here to view our Standard Protocols