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CoraLite® Plus 488-conjugated ITPR1-specific Polyclonal antibody

ITPR1-specific Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19962

Print datasheet

Synonyms

ITPR1, IP3R 1, IP3R, INSP3R1, Inositol 1,4,5-trisphosphate receptor type 1

验证数据展示

  • IF Staining of mouse brain using CL488-19962

    IF Staining of mouse brain using CL488-19962

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 ITPR1-specific antibody (CL488-19962) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-19962

    FC experiment of HepG2 using CL488-19962

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ITPR1-specific (CL488-19962) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 19962-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19962 targets ITPR1-specific in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol 1,4,5-triphosphate receptor, type 1
Synonyms ITPR1, IP3R 1, IP3R, INSP3R1, Inositol 1,4,5-trisphosphate receptor type 1
Calculated Molecular Weight 314 kDa
Observed Molecular Weight 290-300 kDa
GenBank Accession NumberNM_001099952
Gene Symbol ITPR1
Gene ID (NCBI) 3708
RRIDAB_2919168
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14643
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Download protocol
FC protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Download protocol
Standard Protocols
Click here to view our Standard Protocols