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验证数据展示

  • IF Staining of mouse brain using CL488-19962

    IF Staining of mouse brain using CL488-19962

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 ITPR1-specific antibody (CL488-19962) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-19962

    FC experiment of HepG2 using CL488-19962

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ITPR1-specific (CL488-19962) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated ITPR1-specific Polyclonal antibody

ITPR1-specific Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-19962

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

ITPR1, IP3R 1, IP3R, INSP3R1, Inositol 1,4,5-trisphosphate receptor type 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 19962-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19962 targets ITPR1-specific in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol 1,4,5-triphosphate receptor, type 1
别名 ITPR1, IP3R 1, IP3R, INSP3R1, Inositol 1,4,5-trisphosphate receptor type 1
计算分子量 314 kDa
观测分子量 290-300 kDa
GenBank蛋白编号NM_001099952
基因名称 ITPR1
Gene ID (NCBI) 3708
RRIDAB_2919168
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14643
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Download protocol
FC protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Download protocol
Standard Protocols
Click here to view our Standard Protocols