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CoraLite® Plus 647-conjugated ITPR1-specific Polyclonal antibody

ITPR1-specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-19962

Print datasheet

Synonyms

ITPR1, IP3R 1, IP3R, INSP3R1, Inositol 1,4,5-trisphosphate receptor type 1

验证数据展示

  • FC experiment of HepG2 using CL647-19962

    FC experiment of HepG2 using CL647-19962

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ITPR1-specific (CL647-19962) (red), or 0.2 ug CoraLite® Plus 647-conjugated Rabbit IgG Isotype Control (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 19962-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-19962 targets ITPR1-specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol 1,4,5-triphosphate receptor, type 1
Synonyms ITPR1, IP3R 1, IP3R, INSP3R1, Inositol 1,4,5-trisphosphate receptor type 1
Calculated Molecular Weight 314 kDa
Observed Molecular Weight 290-300 kDa
GenBank Accession NumberNM_001099952
Gene Symbol ITPR1
Gene ID (NCBI) 3708
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14643
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 ITPR1-specific antibody CL647-19962Download protocol
Standard Protocols
Click here to view our Standard Protocols