INS Monoclonal antibody
INS Monoclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IHC, IF-P, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
4B6A7
验证数据展示
经过测试的应用
| Positive IHC detected in | human pancreas tissue, mouse pancreas tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas tissue, mouse pancreas tissue |
Planning an IHC experiment? We recommend our IHCeasy Insulin Ready-To-Use IHC Kit. Insulin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66198-1-Ig targets INS in WB, IHC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF, Cell treatment |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | INS fusion protein Ag8630 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | INS |
| Synonyms | insulin, 4B6A7 |
| Calculated Molecular Weight | 110 aa, 12 kDa |
| GenBank Accession Number | BC005255 |
| Gene Symbol | INS |
| Gene ID (NCBI) | 3630 |
| RRID | AB_2881591 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01308 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for INS antibody 66198-1-Ig | Download protocol |
| IF protocol for INS antibody 66198-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Food Chem X Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice. | ||
FASEB J cGAS-STING mediates cytoplasmic mitochondrial-DNA-induced inflammatory signal transduction during accelerated senescence of pancreatic β-cells induced by metabolic stress. | ||
Ecotoxicol Environ Saf Green light exposure aggravates high-fat diet feeding-induced hepatic steatosis and pancreatic dysfunction in male mice. | ||
J Cell Mol Med SDF-1 inhibits the dedifferentiation of islet β cells in hyperglycaemia by up-regulating FoxO1 via binding to CXCR4. |
