CoraLite® Plus 488-conjugated INS Monoclonal antibody

INS Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B6A7

Cat No : CL488-66198

Print datasheet

Synonyms

ILPR, INS, IRDN



经过测试的应用

Positive IF detected inmouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy Insulin Ready-To-Use IHC Kit. Insulin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66198-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-66198 targets INS in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF
Cited Applications IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen INS fusion protein Ag8630 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name INS
Synonyms ILPR, INS, IRDN
Calculated Molecular Weight 110 aa, 12 kDa
GenBank Accession NumberBC005255
Gene Symbol INS
Gene ID (NCBI) 3630
RRIDAB_2883281
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01308
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. It is synthesized as a precursor molecule, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 INS antibody CL488-66198Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Endocrinology

Discordant effects of polyamine depletion by DENSpm and DFMO on β cell cytokine stress and diabetes outcomes in mice

Authors - Batoul Hammoud